Gadsdon's
Alum Hematoxylin
This formula is by Prof. Derek Gadsdon at the Sheffield Children's Hospital, UK.
Ingredient | Amount | Function |
Hematoxylin | 5.5 g | Dye |
Potassium alum | 60 g | Mordant |
Distilled water | 610 mL | Solvent |
100% ethanol | 100 mL | Solvent |
Glycerol | 300 mL | Stabiliser |
Sodium iodate | 0.5 g | Oxidant |
Glacial acetic acid | 20 mL | Acidifier |
Compounding procedure
Dissolve the hematoxylin in 100 mL ethanol.
Dissolve the Alum in 600 mL water.
Dissolve the sodium iodate in 10 mL water.
Combine the hematoxylin and Alum solutions. Mix well.
Add the sodium iodate solution. Mix well.
Add the acetic acid. Mix well.
Leave overnight.
Add the glycerol. Mix well.
Filter before use.
The solution may be used immediately.
It is stable at room temperature for at least 6 months.
Method
Expected results
Notes
Reference
Histopathology laboratory, Red Cross Children's Hospital.
Gadsdon's Haematoxylin Method
Histonet communication, Nov, 1996
Jim Elsam, HTEQA Services.
Gadsdon's Hx
Histonet communication, Nov, 1996