|Potassium alum||50 g||Mordant|
|Distilled water||800 mL||Solvent|
|Sodium iodate||0.2 g||Oxidant|
Dissolve the hematoxylin in the glycerol.
Dissolve the Alum in 750 mL of the water.
Dissolve the sodium iodate in the remaining 50 mL water.
Add the Alum solution to the hematoxylin solution slowly, while mixing well.
Add the sodium iodate solution. Mix well.
The solution may be used immediately, and is stable for about six months.
Bancroft, J.D. and Stevens A. (1982)
Theory and practice of histological techniques, Ed. 2
Churchill Livingstone, Edinburgh & London, UK.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide. p.187.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.