Although there are three variations listed for Anderson's formulas, those marked as 1923a and 1923b are variations of the same solution, the former being that given in the Microtomist's Formulary and Guide and the latter in the Microtomist's Vade Mecum.
|Hematoxylin||2.5 g||2.5 g||5 g||Dye|
|Ammonium alum||20 g||Sat.||30 g||Mordant|
|Distilled water||900 mL||900 mL||700 mL||Solvent|
|95% ethanol||50 mL||50 mL||50 mL||Solvent|
|Calcium hypochlorite||4 g||–||40 g||Oxidant|
|Chloramine T or lime chloride||4 g||Oxidant|
|Glacial acetic acid||50 mL||50 mL||50 mL||Acidifier|
1923a & 1929
Dissolve the calcium hypochlorite in 200 mL water.
Dissolve the hematoxylin in some of the water.
After 4 hours, combine the solutions.
Dissolve the other ingredients in the rest of the water.
Combine with the hematoxylin-hypochlorite solution.
Bring 700 mL water to the boil, then saturate it with alum (see notes).
Allow to cool for one day, then filter
Dissolve the chloramine T or lime chloride into 200 mL of water.
Leave four hours. Shake occasionally.
Dissolve the hematoxylin in the ethanol
Add the oxidant solution to the hematoxylin.
Mix for a few seconds. It should be dark brown.
Slowly add to the 700 mL alum solution, while shaking.
Add the acetic acid.
It is ready immediately.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Bolles Lee, A.. Edited by Gatenby, J.B. and Beams, H.W., (1950)
The Microtomist's Vade-Mecum. 11 ed.,
Churchill, London, UK.
Anderson, J., (1923)
Journal of Pathology and Bacteriology.
Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA