Unna's Orcein-Aniline Blue
|Acetic acid, glacial||1||mL|
|Acetic acid, glacial||2.5||mL|
|Dissolve the aniline blue in the water with heat.|
|Cool and filter.|
|Dissolve the orcein in the ethanol.|
|Add the acetic acid and glycerol.|
|Combine the solutions.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Gatenby, J. B. & Cowdry, E. V., (1928)
Bolles Lee's Microtomist's Vade Mecum, pp. 280
Last updated May 2005.