for Elastic Fibres
|Ferric chloride, 30% aqu.||25||mL|
|Hydrochloric acid, conc.||4||mL|
|Add the dye and resorcin to the water in an oversize flask.|
|Bring to the boil, and add the ferric chloride. Boil for 5 minutes.|
|Cool and filter. Dry the filter paper and beaker.|
|Place the precipitate and filter paper back into the flask.|
|Add the ethanol and heat carefully until the precipitate dissolves.|
|Add the hydrochloric acid. Restore to 200 mL with 95% ethanol.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.
Drury, R.A.B. and Wallington, E.A., (1980)
Carleton's histological technique Ed. 5
Oxford University Press, Oxford, UK.
Humason, G. L., (1967)
Animal tissue techniques, Ed. 2
W. H. Freeman and Company, San Francisco, Ca, USA
Last updated May 2005.