for Elastic Fibres
|Weigert's iron hematoxylin or equivalent|
|Van Gieson's picro-fuchsin|
|Potassium permanganate, 0.25% aqu., for the Mallory bleach|
|Preparing the precipitate|
|Victoria blue 4R||1||g||In an oversized flask heat the water and add the three dyes. When dissolved, add the resorcin, dextrin and ferric chloride solution in the order given. Boil for 5 minutes, then filter while hot.|
|Ferric chloride, 30% aqu.||50||mL|
|Ethanol, 95%||200||mL||Place the precipitate and filter paper back into the original flask. Add the ethanol and simmer gently for 20 minutes. Cool and filter. Restore the volume to 200 mL with 95% ethanol. Add the hydrochloric acid.|
|Hydrochloric acid, conc.||2||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Miller, P.J., (1971)
Journal of Medical Laboratory Technology, V. 28
Miller's Elastic Staining Protocol
Last updated December 2009.