for Elastic Fibres
|Weigert's iron hematoxylin or equivalent|
|Van Gieson's picro-fuchsin|
|Potassium permanganate, 0.25% aqu., for the Mallory bleach|
|Preparing the precipitate|
|Victoria blue 4R||1||g||In an oversized flask, add the dyes to the water, and boil to dissolve. Add the resorcin, dextrin and ferric chloride. Boil for 3 minutes. Cool and filter.|
|Ferric chloride, 30% aqu.||25||mL|
|Ethanol, 95%||100||mL||Place the precipitate and filter paper back into the original flask. Add the ethanol and simmer gently for 15 minutes. Cool and filter. Make up the volume to 350 mL with 95% ethanol. Add the phenol and hydrochloric acid.|
|Hydrochloric acid, conc.||4||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Humberstone, G. C. W., and Humberstone, F. D., (1969)
An Elastic Tissue Stain
Journal of Medical Laboratory Technology, V. 26, No 2, pp. 99.
Last updated December 2009.