Fullmer and Lillie's
Orcinol-New Fuchsin for Elastic Fibres
|Orcinol-New Fuchsin solution|
|New fuchsin||2||g||Add the dye and orcinol to the water in an oversize flask. Boil for 5 minutes. Add the ferric chloride. Boil for a further 5 minutes. Cool and filter. Dry the filter paper and beaker. Place the precipitate and filter paper back into the flask. Add the ethanol and heat carefully until the precipitate dissolves. Restore to 100 mL with 95% ethanol.|
|Ferric chloride, 30% aqu.||50||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Fullmer, H M and Lillie, R D,, (1956).
A selective stain for elastic tissue (orcinol-new fuchsin).,
Stain Technologyl, v 31, page 27-29.
Lillie, R D and Fullmer, H M, (1976).
Histopathological technic and practical histochemistry, Ed. 4. pp. 714.
McGraw-Hill, New York, USA.
Last updated August 2012.