Gabe's Aldehyde Fuchsin
|Add the dye to the water and boil. Cool to room temperature.|
|Add paraldehyde and hydrochloric acid. Ripen at RT two days. Filter.|
|Wash precipitate with 50mL distilled water. Dry the ppt and paper at 60°C.|
|Store in a labelled container. Stable for about ten years.|
|Acetic acid, glacial||2||mL|
|Hydrochloric acid, conc.||1||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Kiernan. J.A., (1999)
Histological and histochemical methods: Theory and practice, Ed. 3
Butterworth Heinemann, Oxford, UK.
Gabe, M., (1976)
Last updated May 2005.