Gomori's Aldehyde Fuchsin
|Hydrochloric acid, conc.
|Dissolve the dye in the ethanol. Add paraldehyde and hydrochloric acid. Ripen at room temperature for 48-72 hours.
Refrigerate. The solution is stable for 2-3 months.
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Other fixatives are likely to be satisfactory.
- Bring sections to water via xylene and ethanol.
- Wash with water.
- Rinse with 70% ethanol.
- Place in the staining solution for 10 minutes.
- Rinse well with 95% ethanol.
- Counterstain the nuclei and/or the cytoplasm if wished.
- Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
- Elastic fibres – purple
- Mast cells – purple
- Pituitary β cells – purple
- Sulphated mucins – purple
- Background – as the counterstain
- Nuclei – as the nuclear stain
- The basic fuchsin used for this solution should be one that is suitable for Schiff's reagent,
i.e., it should have a high pararosanilin content. Both methods involve
forming a compound between an aldehyde and dye.
- Light counterstaining with a progressive alum hematoxylin and
eosin is also suitable.
- Many other counterstains can be used, including methods such as
- Gabe described a technique for the preparation and use of aldehyde fuchsin powder.
Drury, R.A.B. and Wallington, E.A., (1980)
Carleton's histological technique Ed. 5
Oxford University Press, Oxford, UK.
Last updated January 2019