An acid resistant nuclear stain, such as Weigert's iron hematoxylin or the Celestine blue hemalum sequence
|Saturated aqueous Picric acid||100||mL|
|1% aqueous Acid fuchsin||5-15||mL|
Many fixatives, including formalin, are satisfactory.
Stains using acid dyes often benefit from picric acid or mercuric chloride fixation.
Paraffin sections at 5µ are suitable.
This method is often used to counterstain other primary staining methods. In that case the nuclear stain may not be necessary and should be ommitted.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Drury, R.A.B. and Wallington, E.A., (1980)
Carleton's histological technique Ed. 5
Oxford University Press, Oxford, UK.
Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.