for Mallory Bodies
|Weigert's iron hematoxylin or equivalent|
|Dissolve the aniline blue in the acid with gentle heat.|
|Cool. Add the chromotrope 2R. Filter.|
3µ paraffin sections of neutral buffered formalin fixed liver are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Roque, Agustin L., (1953),
Chromotrope aniline blue method of staining Mallory bodies of Laennec's cirrhosis.
Laboratory Investigation, v. 2, no. 5, pp. 15-21.
The reference was provided by Rena Fail.
The procedure was provided by Gundi Lang, Linz General Hospital, Austria.
Last updated May 2005.