for Muscle and Collagen
|Weigert's iron hematoxylin or equivalent|
|Light green SF||0.15||g|
|Acetic acid, glacial||1||mL|
|Mix the water, ethanol and acetic acid together.|
|Divide into four, and dissolve as follows: –|
|· Part 1: phosphomolybdic acid.|
|· Part 2: phosphotungstic acid and orange G.|
|· Part 3: light green SF.|
|· Part 4: ponceau 2R and acid fuchsin.|
|Combine all solutions and filter.|
|Acetic acid, glacial||0.2||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Archives of pathology and laboratory medicine, v. 37, pp. 294
Last updated May 2005.