for Elastic and Collagen
|Solution B – Var I||Var II|
|Eosin B||0.7||g||Eosin B, 2% in 50% ethanol||30||mL|
|Acid fuchsin, sat. aqu.||4||mL||Acid fuchsin, sat. aqu.||4||mL|
|Unna's elastic stain||35||mL||Unna's elastic stain||30||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Gatenby, J. B. & Cowdry, E. V., (1928)
Bolles Lee's Microtomist's Vade Mecum, pp. 280
Bolles Lee, A.. Edited by Gatenby, J.B. and Beams, H.W., (1950)
The Microtomist's Vade-Mecum. 11 ed., pp. 485
Churchill, London, UK.
Last updated May 2005.