Paquin & Goddard's Trichrome
for Elastic and Collagen
|Paquin & Goddard's iron hematoxylin or equivalent|
|Acetic acid, glacial||4||mL|
|Acetic acid, glacial||10||mL|
5µ paraffin sections of Masson's picric-chrome-formalin fixation was specified. Most trichrome stains benefit from picric acid or mercuric chloride fixation. The specified fixation may be compensated for by secondary fixation of sections in Bouin's fluid.
|Picric acid, sat. aqu.||187.5||mL||Add the alum to the formalin and leave for 1 hour.|
|Chromium aluminum sulphate||7.5||g||Add the picric acid solution.|
|Formalin, Conc. 40%||67.5||mL||Let stand for 24 hours, and filter.|
Return to fixation.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Paquin & Goddard, (1947)
Bulletin of the International Association of Medical Museums and
Journal of Technical Methods, v.27, pp.198
Last updated May 2005.