for Muscle and Collagen
|Hydrochloric acid, conc||2.25||mL|
|Acetic acid, glacial||1||mL|
|Fast green FCF||0.1||g|
|Acetic acid, glacial||0.2||mL|
5µ paraffin sections of neutral buffered formalin fixed were specified. Other fixatives may be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Technical Bulletin, v. 7, pp.57
Last updated May 2005.