3rd Variant for Muscle and Collagen
|Regaud's iron hematoxylin or equivalent|
|Acetic acid, glacial||2||mL|
|Acetic acid, glacial||2.5||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
|Solution A||Solution D||Solution F|
|Picric acid, sat. alc.||12||mL||Picric acid, sat. alc.||12||mL||Picric acid, sat. alc.||6||mL|
|Ethanol, 95%||68||mL||Ethanol, 95%||68||mL||Ethanol, 95%||14||mL|
|Distilled water||20||mL||Distilled water||20||mL||Distilled water||80||mL|
|Orange G||0.25||g||Phosphotungstic acid||10||g||Phosphotungstic acid||5||g|
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Stain Technology, v. 19, pp. 23
Last updated May 2005.