Lewis and Miller's Trichrome
for Pituitary cells
This is a modification of Kricheski's method
|Methyl blue, 1% aqueous||30||mL|
|Orange G, 1% aqueous||30||mL|
|Phosphomolybdic acid, 1% aqueous||30||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are likely suitable. Other fixatives are also likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Stain technology, v.6, pp.97
Lewis and Miller, (1938)
Stain technology, v.14, pp.111
Last updated May 2005.