Lendrum and McFarlane's
Trichrome for Muscle and Collagen
Solutions
Solution A | ||
Picric acid | 1 | g |
Orange G | 0.2 | g |
Ethanol, 95% | 80 | mL |
Water | 20 | mL |
Solution B | ||
Acid fuchsin | 0.5 | g |
Ponceau 2R | 0.5 | g |
Sodium sulphate | 0.25 | g |
Acetic acid, glacial | 1 | mL |
Distilled water | 99 | mL |
Solution C | ||
Acetic acid, glacial | 1 | mL |
Distilled water | 99 | mL |
Solution D | ||
Phosphomolybdic acid | 1 | g |
Distilled water | 100 | mL |
Solution E | var I | var II | ||||
Aniline blue | 2 | g | – | |||
Fast green FCF | – | 2 | g | |||
Acetic acid, glacial | 1 | mL | 1 | mL | ||
Distilled water | 100 | mL | 100 | mL |
Tissue sample
Most trichrome stains benefit from picric acid or mercuric chloride fixation.
5µ paraffin sections of neutral buffered formalin fixed tissue would probably be suitable, although
secondary fixation of sections in Bouin's fluid overnight or at 56°C for an hour would undoubtedly enhance staining.
Method
Expected results
Notes
Solution A | ||
Picric acid, sat. ethanolic | 12 | mL |
Orange G | 0.2 | g |
Ethanol, 95% | 68 | mL |
Water | 20 | mL |
Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Citing:–
Lendrum and Mcfarlane, (1940)
Journal of Pathology and Bacteriology, v. 50, pp. 381
London, UK.