Kricheski's Trichrome
for Muscle and Collagen

Solutions

Solution A
Acid fuchsin 0.25 g
Distilled water 100 mL
Solution B
Methyl blue, 1% aqueous 30 mL
Orange G, 1% aqueous 30 mL
Phosphomolybdic acid, 1% aqueous 30 mL

Tissue sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.

Method

  1. Bring sections to water via xylene and ethanol.
  2. Place into solution A for 1-3 minute.
  3. Rinse well with distilled water.
  4. Place into solution B for 3-5 minutes.
  5. Dip 2 or 3 times in 70% ethanol.
  6. Dehydrate and differentiate with ethanol for 1-3 minutes.
  7. Clear with xylene and mount with a resinous medium.
  8. Bring sections to water via xylene and ethanol.

Expected results

Notes

  1. Although this method does not specify that an acid resistant nuclear stain be used, Weigert's iron hematoxylin or equivalent could be inserted before step 2, lightly differentiated and blued. The nuclei would then be black.

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Kricheski, (1931)
    ?? Stain technology, v.6, pp.97
    Gray gives references by assigning a number to each journal.
    The number given for this reference is not identified with a journal.
    However, Stain technology's number differs only by the first digit

 


 

 

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