for Muscle and Collagen
|Böhmer's alum hematoxylin|
|Ferric ammonium sulphate||5||g|
|Dissolve the iron alum into 25 mL of the water.|
|Combine the remaining ingredients.|
|Mix the two solutions together and filter.|
Zenker fixation was recommended, and 5µ paraffin sections would be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation.
Formalin fixation could probably be used with secondary fixation of sections in Bouin's fluid.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Bulletin of the International Association of Medical Museums
and Journal of Technical Methods., v. 6, p. 61
Montreal, Que. Canada & Washington, DC, USA
Last updated May 2005.