for Muscle and Collagen
|Weigert's iron hematoxylin or equivalent|
|Fast green FCF||0.3||g|
|Acetic acid, glacial||1||mL|
|Acetic acid, glacial||0.2||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Technical Bulletin, vol.20, pp.77
Technical Bulletin, vol.21, pp.92
Humason, G. L., (1967)
Animal tissue techniques, Ed. 2
W. H. Freeman and Company, San Francisco, Ca, USA
Last updated May 2005.