Engel & Cunningham's Trichrome
for Muscle Fibres, Types I & II
|Harris alum hematoxylin|
|Fast green FCF||0.3||g|
|Acetic acid, glacial||1||mL|
|Adjust to pH 3.4 with 1N NaOH.|
|Acetic acid, glacial||0.2||mL|
Unfixed frozen sections of liquid nitrogen quenched muscle. Sections should be 5-10µ in thickness. The fibres should be in cross section, as close to a right angle to their length as possible.
Culling, C.F.A., Allison, R.T. and Barr, W.T.
Cellular Pathology Technique, Ed.4.
Butterworth, London, UK.
Engel & Cunningham, (1963)
Rapid examination of muscle tissue.
An improved trichrome method for fresh frozen biopsy specimens.
Neurology, vol.13, pp.921
Last updated May 2005.