for Muscle and Collagen
|Add about 20 g dye to the aniline water.|
|Shake intermittently for 48 hours. Filter.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Mallory, F. B., (1938),
Saunders, Philadelphia, Pa, USA
Last updated May 2005.