Orange-Fuchsin-Green for Pituitary
|Acetic acid, glacial||0.5||mL|
|Light green SF yellowish||1.5||g|
|Acetic acid, glacial||1.5||mL|
Although 5µ paraffin sections of neutral buffered formalin fixed tissue are likely adequate, superior results are obtained with fixation formal sublimate. Other fixatives may be satisfactory. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Last updated May 2005.