for Cellular Inclusions
This method is also known as the Rhoda-Coomassie.
|Celestine blue-hemalum nuclear stain, or similar.|
|Lissamine rhodamine B||1||g|
|Acetic acid, glacial||2.5||mL|
|Coomassie fast yellow G||to||saturation|
5µ paraffin sections of neutral buffered formalin fixed tissue are likely suitable. It should be noted, however, that the authors of this method favoured extended fixation in formal sublimate of up to 10 days for fibrin. This fixative is now deprecated due to its mercuric chloride content. With other staining methods, pretreatment of sections with Bouin's fluid for an hour at 60°C can compensate for the lack of mercury fixation to a large degree.
Lendrum A C, Fraser D S, Slidders W and Henderson R. (1962)
Studies on the character and staining of fibrin.
Journal of clinical pathology, v. 15, p. 401.
Last updated January 2010.