Orange-Fuchsin-Green for Pituitary
|Acetic acid, glacial||0.5||mL|
|Light green SF yellowish||1.5||g|
|Acetic acid, glacial||1.5||mL|
Although 5µ paraffin sections of neutral buffered formalin fixed tissue are likely adequate, superior results are obtained with fixation in formal sublimate, preferably about 48 hours. Other fixatives may be satisfactory. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Putt, F. A.
Manual of Histopathological Staining Methods
John Wiley & Sons, New York, NY., USA
Slidders, W. J., (1961)
Journal of Pathology and Bacteriology, v. 82, p. 532
Last updated Sep 2005.