Roque's Stain
for Cell Inclusions
Solutions
Stock solution A | ||
Citrate buffer, pH 5.8 | 100 | mL |
Methyl green, purified | 0.1 | g |
Thionin | 0.0165 | g |
Dissolve the thionin in a small amount of water. Add the buffer and methyl green. Shake and filter. Use fresh. |
Citrate buffer, pH 5.8 | ||
Hydrochloric acid, 0.01M | 42 | mL |
Sodium citrate, 0.01M | 58 | mL |
Dehydrant | ||
Tertiary butanol | 80 | mL |
Ethanol, absolute | 20 | mL |
Tissue sample
Fix 2mm thick pieces of tissue in 10% formalin containing 1% sodium acetate for 3 hours. Fix smears with methanol.
Method
Expected results
Notes
Reference
Humason, G. L., (1967).
Animal Tissue Techniques., pp. 278
W. H. Freeman and Company, San Francisco, CA, USA.