for Cell Inclusions
|Stock solution A|
|Citrate buffer, pH 5.8||100||mL|
|Methyl green, purified||0.1||g|
|Dissolve the thionin in a small amount of water. Add the buffer and methyl green. Shake and filter. Use fresh.|
|Citrate buffer, pH 5.8|
|Hydrochloric acid, 0.01M||42||mL|
|Sodium citrate, 0.01M||58||mL|
Fix 2mm thick pieces of tissue in 10% formalin containing 1% sodium acetate for 3 hours. Fix smears with methanol.
Humason, G. L., (1967).
Animal Tissue Techniques., pp. 278
W. H. Freeman and Company, San Francisco, CA, USA.
Last updated March 2016.