Pappenheim Stain
for Plasma Cells

Solutions

Stock solution A
Methyl green 1 g
Distilled water 100 mL
Phenol 0.25 g
Stock solution B
Pyronin Y 4 g
Distilled water 100 mL
Phenol 5 g
Working solution
Stock solution A 15 mL
Stock solution B 35 mL

Tissue sample
Most fixatives should be satisfactory.

Method

  1. Bring sections to water via xylene and ethanol.
  2. Place into the staining solution for 5-10 minutes.
  3. Rinse briefly with water.
  4. Dehydrate rapidly with acetone.
  5. Clear with xylene and mount with a resinous medium.

Expected results

 

Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide. p. 355
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.