Nissl's Methylene Blue
for Nissl bodies
|Dissolve the soap in the water.
Add and dissolve the dye.
Allow to ripen at least three months
Ethanol fixation is preferred. Formalin fixed tissue may be suitable. Sections should be thicker than usual, and were originally to be free floating sections, likely free hand sections prepared without freezing.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide. pp. 446.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Gatenby, J.B. and Beams, H.W., (1950)
The Microtomist's Vade-Mecum. 11 ed., pp. 508, para. 1099
Churchill, London, UK.
Last updated April 2016.