Compare Phenolic MGP
Solutions

Solutions

Ingredient   Pappenheim Unna Sandiford Scott & French Langeron Kurnick
Distilled water
Glycerol
Ethanol, 100%
Ethanol, 95%
Phenol
Methyl green
Pyronin Y
mL
mL
mL
mL
g
g
g
100



0.25
0.3
0.7
82
16
2

0.4
0.12
0.2
75
20

5
1.5
0.15
0.5
80
16
4

1.6
0.8
0.2
100



5
2
2
100




0.6
1

Tissue sample
Most fixatives should be satisfactory.

Method
These solutions have been included for comparison purposes, and the formulae have been adjusted to make 100 mL of each solution to facilitate the comparison. See the individual methods for details of their use and the actual formula. There is no single method for their application, but the details below may be used as a starting point.

  1. Bring sections to water via xylene and ethanol.
  2. Place in the MGP solution for 10 minutes at room temperature.
  3. Rinse with distilled water.
  4. Differentiate with absolute ethanol if required.
  5. Complete dehydration with acetone if necessary.
  6. Clear with xylene and mount with a resinous medium.

Expected results

Notes

  1. The time and temperature in step 2 may need to be increased.
  2. Differentiation may not be required, depending on the time and temperature of staining.
  3. Various fluids have been used for dehydration: acetone, n-butanol, t-butanol, etc.

Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide. p. 355
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.

Disbrey, B. D., (1970)
Histological laboratory methods.
E. & S. Livingstone, Edinburgh and London, UK.