Langeron's Stain
for Cell Inclusions

Solutions

Stock solution A
Distilled water 100 mL
Methyl green 4 g
Phenol 5 g
Stock solution B
Distilled water 100 mL
Pyronin Y 4 g
Phenol 5 g
Working solution
Stock solution A 25 mL
Stock solution B 25 mL
Differentiator
Ethanol, absolute 25 mL
Acetone 25 mL

Tissue sample
Most fixatives should be satisfactory.

Method

  1. Bring sections to water via xylene and ethanol.
  2. Place into the staining solution for 15 minutes at 50°C.
  3. Rinse briefly with distilled water.
  4. Differentiate until staining is clear.
  5. Dehydrate with amyl alcohol.
  6. Clear with xylene and mount with a resinous medium.

Expected results

Notes

 

Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide. p. 355
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.