Kurnick Stain
for Plasma Cells

Solutions

Stock pyronin
Pyronin Y 2 g
Distilled water 100 mL
Stock methyl green
Methyl green 2 g
Distilled water 100 mL
Working solution
Stock pyronin 12.5 mL
Stock methyl green 7.5 mL
Distilled water 30 mL

Storing stock solutions
The pyronin stock solution should be extracted with chloroform ten times to ensure purity. The methyl green stock solution should be extracted a minimum of six times, until the chloroform is no longer coloured with any crystal violet. Store both solutions over a layer of chloroform.

Tissue sample
Most fixatives should be satisfactory if fixation time is not extended, but avoid potassium dichromate or picric acid. Overnight with a 10% formalin variant should be satisfactory.

Method

  1. Bring sections to distilled water via xylene and ethanol.
  2. Place into the staining solution for 6 minutes in a coplin jar.
  3. Blot gently.
  4. Dehydrate with n-butanol, two changes of 5 minutes each.
  5. Clear with xylene and mount with a resinous medium.

Expected results

Notes

Reference
Disbrey, B. D., (1970)
Histological laboratory methods. pp.187-188.
E. & S. Livingstone, Edinburgh and London, UK.