for Plasma Cells
|Stock methyl green|
|Stock methyl green||7.5||mL|
Storing stock solutions
The pyronin stock solution should be extracted with chloroform ten times to ensure purity. The methyl green stock solution should be extracted a minimum of six times, until the chloroform is no longer coloured with any crystal violet. Store both solutions over a layer of chloroform.
Most fixatives should be satisfactory if fixation time is not extended, but avoid potassium dichromate or picric acid. Overnight with a 10% formalin variant should be satisfactory.
Disbrey, B. D., (1970)
Histological laboratory methods. pp.187-188.
E. & S. Livingstone, Edinburgh and London, UK.
Last updated April 2016.