|Alum hematoxylin, such as Mayer (Langeron).|
|Carmine||1||g||Combine the carmine, aluminum chloride and water.|
|Aluminum chloride||0.5||g||Heat until the colour changes to a deep maroon red.|
|Distilled water||3||mL||Cool, and add the 50% ethanol. Leave 24 hours before use.|
|Ethanol, 50%||100||mL||Make every 7 - 10 days.|
Alcohol fixed tissues were originally recommended, but 5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Mitteilungen aus der Zoologischen Station zu Neapel, v. 12, p. 303
Bensley R. R. and Bensley, S. H., (1938)
Handbook of Histological and Cytological Technique.
U. Chicago Press, Chicago, USA
Last updated Sep 2005.