Vassar & Culling's Thioflavine T
for Amyloid Fluorescence
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Using a UG1 or UG2 exciter filter and a UV barrier filter, or
a BG12 exciter and an OG4 or OG5 barrier filter,
Amyloid fluoresces bright yellow.
Vassar, P.S. and Culling, C.F.A., (1959),
Fluorescent stains with special reference to amyloid and connective tissue,
Archives of pathology, v 68, page 487
Bancroft, J.D. and Stevens A. (1982)
Theory and practice of histological techniques Ed. 2
Churchill Livingstone, Edinburgh & London, UK.
Last updated May 2005.