Lynch & Inwood's Gold
for Amyloid

Solutions

Chloro-auric acid, 1% aqueous
Hydrogen peroxide, 3% aqueous
Iodine solution
Iodine 1 g
Potassium iodide 2 g
Distilled water 100 mL

Mix the potassium iodide and iodine crystals together. Add 5 mL of the water and mix until both have dissolved. Add the rest of the water.

Tissue sample
5-10µ sections from formalin fixed, paraffin embedded tissues, mounted on slides are suitable.

Method

  1. Bring sections to water through xylene and ethanols.
  2. Place in the iodine solution for 2½-5 minutes.
  3. Rinse with distilled water, 3 times of 5-10 seconds each.
  4. Place in chloro-auric acid solution for 2½-5 minutes.
  5. Rinse with distilled water, 3 times of 5-10 seconds each.
  6. Place in fresh 3% hydrogen peroxide at 37°C for 6-36 hours.
  7. Dehydrate with ethanol, clear with xylene and mount using a resinous medium.

Expected results

Notes

  1. Chloro-auric acid is also known as brown gold chloride.
  2. Overnight is usually sufficient for treatment with hydrogen peroxide. The length of time may be reduced by adding 0.2 mL of a 0.1N sodium hydroxide solution to 100 mL of 3% hydrogen peroxide and treating the sections with it for 3 to 6 hours.

Reference
Lynch, M.J. and Inwood, M.J.H., (1963),
Gold as a permanent stain for amyloid,
Stain Technology, v 38, page 260.

 


 

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