Highman's Crystal Violet
|· Weigert's iron hematoxylin|
|· Highman's modification of Apathy's gum syrup|
· Crystal violet
|Glacial acetic acid||2.5||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Cryostat sections usually show brighter metachromasia. Unmounted frozen sections may also be floated in each solution and mounted on a slide just before coverslipping.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide, p. 452.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Last updated April 2012.