Crystal Violet for Amyloid
Frozen sections are preferred. Cryostat sections usually show brighter metachromasia.
Unmounted frozen sections may also be floated in each solution and mounted on a slide just before coverslipping.
5µ paraffin sections of neutral buffered formalin fixed tissue are likely also suitable.
Other fixatives may be satisfactory.
- Bring sections to water via xylene and ethanol, except for cryostat and frozen sections.
- Place into bismarck brown solution for 5 minutes.
- Rinse well with 95% ethanol, then rinse with distilled water.
- Place into crystal violet solution for 5 minutes.
- Rinse with water.
- If necessary, differentiate in 1% acetic acid until amyloid is red and contrasts well with the tissue.
- Wash well in tap water.
- Drain all water from the slide until just damp and mount with levulose syrup.
- Ring the coverslip to inhibit evaporation of the mounting medium.
- Amyloid – purple-red
- Background – Blue-violet
- Nuclei – Brown
- Methyl violet may be used instead of crystal violet if preferred.
- Although levulose syrup (fructose syrup or high fructose corn syrup) is specified it is likely that Highman's gum syrup would be preferable as it inhibits leaching of the dye.
- Although bismarck brown is metachromatic (yellow metachromasia), it is used here as a basic dye for staining nuclei.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide, p.451.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Last updated January 2019