Bancroft's Crystal violet
|· Crystal violet||1% aqueous|
|· Acetic acid||1% aqueous|
|· Methyl green||2% aqueous, washed with chloroform to remove crystal violet|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Cryostat sections usually show brighter metachromasia. Unmounted frozen sections may also be floated in each solution and mounted on a slide just before coverslipping.
Bancroft, J. D., (1963).
Stain technology, v. 38, p. 336.
Last updated April 2012.