Rapid Automatic Processing
In the schedule below, it is presumed that the working day is from 8:00 a.m. to 5:00 p.m.. If other than that, appropriate adjustments should be made.
|Thickness||As thin as practical, usually about 1 mm maximum. Thicker tissues may not be properly processed.|
|Area||10 mm × 15 mm. Thin slices that have too large an area will tend to curl, making sectioning the full surface difficult. Submit more than one piece if a larger area is needed.|
|Fixed tissue||Breadloaf large organs and store in NBF for 48 hours. Select tissue from fixed areas and trim to size.|
|Unfixed tissue||Trim to size and fix at least 2 hours with preheated NBF at 65°C. in a closed container, or fix with a rapid fixative (e.g. Carnoy)|
|Times||This is a rapid and minimalist processing schedule.|
|Clearing agent||Xylene or another clearing agent that will clear tissues in similar times should be used.|
|Processing time||This schedule requires 5 hours after fixation.|
It has been presumed that the processor has 12 stations. If it has more than 12, increase the number of absolute ethanol stations first as clearing and wax infiltration proceed faster with complete dehydration. If it is possible to elevate temperatures, do so in all stations.
It should be understood that this processing schedules is a guide only, and the times given may need to be altered to take the type of tissues being processed into account, as well as other local conditions.