Randolph's Fluid

Solution A
Chromium trioxide 10 g
Acetic acid, glacial 70 mL
Distilled water 930 mL
Solution B
Strong formalin 300 mL
Distilled water 700 mL
Mix equal parts of solutions A and B just before use.

Randolph's fluid is a modification of Navishin's fluid and is somewhat weaker, but more convenient to prepare. It is used for the same purpose, i.e. chromosomes. It is sometimes referred to as "Craf" or "CRAF", both of which refer to "chromium, acetic, formalin", the three active ingredients.

Fixation of average sized pieces is complete after 12 to 24 hours, and then they must be washed very well with running tap water to remove all traces of chromium trioxide.

 

Reference
Davenport, H.A., (1960),
Histological and histochemical techniques,
W. B. Saunders Co., London, UK., Oxford, UK.

 


 

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