Recommended for chromosomes and the Feulgen reaction for DNA. It is said to preserve mucopolysaccharides well.
Since this mixture contains suspect carcinogens and ethers, treat with caution and use in a fume chamber.
A few hours to overnight, depending on tissue thickness.
Transfer to absolute ethanol or clearant.
Handbook of Histopathological and Histochemical Techniques, Ed. 3,
Butterworths, London, UK.