Müller's Fluid

Müller's fluid
Potassium dichromate   25 g
Potassium sulphate 10 g
Distilled water 1 L

Müller's fluid is basically a simple dichromate solution with an indifferent salt (potassium sulphate). Used as the primary fixative it has limited application as the preservation is merely adequate, but it may be used as a secondary fixative for post chroming.

Time
Generally, one or two days fixation for normal slices of tissue should be satisfactory. When used for post chroming it may be applied for several days.

Aftertreatment
Wash well with running tap water to remove excess dichromate.

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Müller, (1859)
    Verhandlungen der Physikalisch-medizinischen Gesellschaft zu Würzburg.

 


 

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