Methacarn

Methacarn
Methanol, absolute 60 mL
Acetic acid, glacial   10 mL
Chloroform 30 mL

Methacarn is Carnoy's fluid in which methanol replaces the ethanol. This causes less shrinkage of the tissue so that morphological preservation is superior to Carnoy. It also preserves antigenic sites more effectively than does Carnoy or other ethanol fixatives. It has been recommended for studies with DNA and RNA.

Time
4 hours or longer.

Aftertreatment
Transfer to absolute ethanol.

Reference
Puchtler, H., Sweat Waldrop, F., Meloan, S.N., Terry, M.S. and Conner, H.M. (1970)
Methacarn (methanol-Carnoy) fixation
Journal of Histochemistry and Cell Biology, v. 21, N° 2., June 1970.

 


 

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