Mann's fluid
| Solution A | ||
| Distilled water | 100 | mL |
| Osmium tetroxide | 1 | g |
| Solution B | ||
| Distilled water | 100 | mL |
| Sodium chloride | 0.75 | g |
| Mercuric chloride | to saturation | |
| Combine equal parts of solutions A and B just before use. The working solution is stable for a few days. |
This solution is used for cytological components. Kiernan notes that it is suitable for the Golgi apparatus. It should fix fairly rapidly, a few to several hours, and should be washed afterwards to remove excess osmium tetroxide.
Reference
Kiernan, J.A.,
Histological & Histochemical Methods, Ed. 3,
Butterworth Heinemann, Oxford, UK.
