Mann's fluid

Solution A
Distilled water 100 mL
Osmium tetroxide 1 g
Solution B
Distilled water 100 mL
Sodium chloride 0.75 g
Mercuric chloride to saturation
Combine equal parts of solutions A and B just before use.
The working solution is stable for a few days.

This solution is used for cytological components. Kiernan notes that it is suitable for the Golgi apparatus. It should fix fairly rapidly, a few to several hours, and should be washed afterwards to remove excess osmium tetroxide.

 

Reference
Kiernan, J.A.,
Histological & Histochemical Methods, Ed. 3,
Butterworth Heinemann, Oxford, UK.

 


 

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