|Acetic acid, glacial||2||mL|
|Osmium tetroxide, 1% aqueous||8||mL|
Dissolve the chromium trioxide in the water, then add the acetic acid. Just before use add the osmium tetroxide.
This is a modification of Flemming's strong fluid. It is used for cytological preservation, chromosomes particularly. As with all of these osmium tetroxide containing fluids, the tissue should be 2 mm or so thick, no more.
Pieces less than 2 millimetres should be fixed within 24 hours.
Wash in running tap water overnight.
Note that these solutions do not store well, and enough should be made for a few days only.
Davenport, H.A., (1960),
Histological and histochemical techniques,
W. B. Saunders Co., London, UK., Oxford, UK.