Carnoy's Fluid

Carnoy's fluid
Ethanol, absolute 60 mL   Prepare just before use
Acetic acid, glacial   10 mL
Chloroform 30 mL

Carnoy's fluid is a very rapid fixative and has been used frequently for fixation when rapidly processed paraffin sections are wanted. For a 1-2 mm piece of tissue only 30 to 60 minutes is required, and dehydration is taking place at the same time. It is a good nuclear fixative, but produces considerable shrinkage and cytological distortion. As with most alcoholic fixatives, glycogen is preserved well.

This is the fixative usually meant when "Carnoy's fluid" is specified. There is another fixative often ascribed to Carnoy, a 3:1 mixture of ethanol and acetic acid, but that is more correctly referred to as Clarke's fluid.

Time
30 minutes to 2 hours.

Aftertreatment
Transfer to absolute ethanol.

Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Carnoy (1887), Cellule, v. 3, p. 276.

 


 

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