Behrens’ Fixative

Water 1 L
Cupric chloride 2 g
Cupric nitrate 2 g
Phenol 10 g
Acetic acid, glacial 10 mL

Fixation time should be determined by trial.

Copper fixatives are not often used in modern histology. They have a reputation for good preservation, as the copper present may act as a mordant for some dyes, including hematoxylin. For that reason nuclear staining may be enhanced.

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Mayer, (1920)
    Zoomikrotechnik
    Berlin, Germany

 


 

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