The formula specifies that the stong formalin should be neutralised. Using a freshly purchased solution may avoid this, but if it does not neutralise by layering the strong formalin over calcium carbonate (marble chips or crushed oyster shells) overnight in a loosely covered container under a fume hood. Never do this in a closed container. Enough carbon dioxide can be generated for the pressure to break a glass jar.
This fixative is useful for the Golgi apparatus and general morphology. Average sized pieces should be fixed within 4 to 12 hours. There is no special aftertreatment.
McManus, J.F.A. and Mowry, R.W., (1960),
Staining methods, histologic and histochemical,
Harper & Row, New York, NY, USA.