Duboscq-Brasil

Picric acid	1	g		Combine the reagents in the order given.
Ethanol, 80%	150	mL
Formalin, concentrated	60	mL
Acetic acid, glacial	15	mL

No details are given in the references as to fixation time and quality. Since the solution is similar to alcoholic Bouin's fluid it should have similar characteristics, i.e. it is likely to be fairly rapid with some hardening, and promotion of staining with acid dyes.

Picric acid is explosive in the dry state, however it saturates at 8.96% in absolute ethanol. 11.2 mL of this solution contains fractionally over 1 gram of picric acid. The formula below closely approximates the one above, but avoids weighing dry picric acid.

Picric acid, saturated alcoholic	11	mL
Ethanol, absolute	109	mL
Water	30	mL
Formalin, concentrated	60	mL
Acetic acid, glacial	15	mL

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Brasil, (1904)
    Archives de zoologie expérimentale et générale,, vol. 2, p. 445

Shokeir, A. A., and Elbagoury, S. M.
Alloxan-Schiff reagent for the detection of fixed rabies virus in the nerve cell
J. med. Lab. Tech. (1966) vol. 23, p. 191